FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43649890
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA746350.17098553971155483No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG661120.15145971730971142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG96450.015.81776738
AGCTTAG96550.015.50537945
GGCATAG103250.014.48749835
CATAGGG105500.014.28830637
GCATAGG116250.012.92733736
GAGCTTA123700.012.24766644
AGGGATC161950.012.0218252
TGGCATA130050.011.58638934
GTCACTC175400.011.584948
GGTCACT184300.011.0621357
ACTCTCC195700.010.53346311
AGGTCAC208650.010.450756
GGGAGGT215100.010.3597433
TAGGGGA194850.010.338757539
CAGGGAT218450.010.2106271
GGAGGTC218500.010.0444414
GCATTCG50950.010.0284983
GCGTAGG52100.09.84515836
ATTTCTA373100.09.8211972
GGGATCA211900.09.634533
Produced by FastQC (version 0.11.5)