FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40070874
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG601830.15019138339732743No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA535440.13362323966280346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC83500.016.306265
AGCTTAG119800.015.92505145
ATAGCTC84900.014.62716539
GGCATAG129950.014.55887835
TCACCCG98650.013.9167293
CATAGGG141500.013.64080937
ATAGGGG143400.013.42898338
ACGGACA98100.013.34917118
CGGACAG97700.013.33473319
GAGCTTA144850.013.32649744
CGGACAC102500.012.97481913
TAGCTCT96150.012.70516740
CCGGACA104350.012.70171112
GCATAGG151650.012.66845436
TGGCATA153350.012.58650634
AGGGATC143300.012.53317452
CACCCGG113100.012.1583124
ACACGGA107550.012.1345116
GTCACTC205250.012.0823778
TGATAGC105050.011.97085137
Produced by FastQC (version 0.11.5)