FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46279371
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG722100.1560306426809474No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG704910.15231624474757877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA206150.020.4268723
ACGCTAC209550.020.05687924
CGCTACC212200.019.6682725
TTCGACG21400.019.1212226
AAAACGC230500.018.39817421
AAACGCT239600.017.72141522
GAAAACG242450.017.51526320
TCGACGA25300.016.973487
CCGCGTT62500.016.8364721
CGGTATA35300.016.01017820
CGTGGTT264150.015.97920641
TCGTGGT264450.015.88047340
GTTTTCG268200.015.57006645
CCCGTAT30650.015.4054381
GCTACCT290150.014.75660226
CCGGCTT303350.014.6675632
CTACCTC292550.014.62012927
CGACGAG26850.014.5700538
CCTCGTG299200.014.39853138
GACCGCG29650.014.1040695
Produced by FastQC (version 0.11.5)