FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49772075
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1741900.34997536269082613No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG592610.11906475669338681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG44350.027.8511586
CGGTATA64750.026.02660420
AGACCGC88250.024.5009944
GTTCGAC51100.024.1716565
CGACGAG51250.023.7050028
TCGACGA52650.023.416547
GACCGCG93900.022.9058935
CAGACCG98100.022.4563793
ACCGCGT106300.020.2766766
TTCGGCG83300.018.63533644
GTATAGA98850.018.32363722
CTACACG78800.018.10071445
AACGACG34300.017.90587416
TCGGCGC88600.017.62213145
CGGCAGT493550.017.54142840
AGTTCGA72700.017.485944
CCGTACG90300.017.3169774
CGTACGC91200.017.1452925
ACGAGGA92900.016.97627410
GACGGTA100700.016.95716718
Produced by FastQC (version 0.11.5)