FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31329922
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT384500.12272612743817236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG76050.027.419455
AGACCGC76100.027.3424934
ACCGCGT78650.026.5416266
CAGACCG83800.025.0717053
CCGCGTT101150.020.5709047
TCAGACC108700.020.1148932
CGCGTTC114150.018.2084758
CTCAGAC148250.017.237021
CGTACGC45700.016.2925875
CCGTACG46150.015.9875964
TTCGACG10750.015.4846546
GCGTTCT141750.014.7583389
TCCGTAC51000.014.5554233
TACGCCA55800.013.7869897
CTCGGTT53100.013.52561951
GACGAAA27850.013.41127245
GCACTAC52900.012.80864425
CGCGCAA3051.6734703E-1012.5490291
GTACGCC60800.012.357236
CCCGTAT20000.012.2703661
Produced by FastQC (version 0.11.5)