FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31329922
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG55100.013.19662138
CGTGGCG70800.012.9286241
CGACCCG77250.011.9291876
GTTGTCG16650.011.8874126
GCGTTCG23550.011.45853615
ACCGGTA17650.011.34545645
GGCATAG64550.011.29669435
AGCTTAG66600.011.11471545
GCGACCC82450.011.0476285
GTGGCGA80200.010.9354552
ATATAGC23700.010.9062396
ATCCTAG58950.010.6376536
GGCGTAG36500.010.54401135
TATCCTA51650.010.52022655
TGGCGAC84750.010.4818943
TCTATAG135900.010.3525743
CGCGTTC27150.010.27095614
CATAGGG71600.010.21828537
CGTAGGG36300.010.10854537
CCGCGTT28450.010.03814213
Produced by FastQC (version 0.11.5)