FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47463387
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC800230.16859943012495082No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA733670.15457598927779848No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG501590.1056793523816579No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG476360.10036367611102005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36150.022.33918426
TGTCGTT43900.018.39553628
ATATAGC52100.017.2820226
TTGTCGT51550.015.88379727
GCATTCG52900.015.8772993
TCGTTCC53800.015.51145830
GCTAAGT56100.015.36183114
TATAGCT62000.015.175817
AGGGATC142050.014.7097562
GTCGTTC56000.014.70153729
CGTTCCC58800.013.96388631
ATTCGGG65050.013.6031195
GGGAGGT271900.013.565263
AATATAG79950.013.208325
GGGATCA164300.013.1295973
TACAATA74750.012.952572
GGAGGTC287400.012.84114554
TTCGGGC71000.012.6181896
CAATATA80400.012.37877754
ATAGGGG190200.012.26491438
Produced by FastQC (version 0.11.5)