FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22822463
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG826120.36197670689618383No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT536160.2349264406738221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG126400.026.435493
AGACCGC129200.025.618764
CTCAGAC154750.023.3592011
GACCGCG152050.021.6796135
GCCGGCT211150.021.5222821
CCGGCTT231450.020.5542412
TCAGACC174750.020.346682
AACGCTA253700.020.15815423
ACGCTAC254700.020.0790124
ACCGCGT166250.020.0024266
CGCTACC257500.019.79951325
CGTGGTT254300.019.78362341
GTTTTCG256400.019.3671945
TCACGCT272250.019.346638
CCGCGTT172100.019.309567
AAAACGC268800.019.11774821
TTCACGC276450.019.0771627
GAAAACG266550.019.02589620
AAACGCT269600.019.00268422
GCGTTCT175100.018.8501919
Produced by FastQC (version 0.11.5)