FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45372867
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2784970.6137963466139356No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1597160.35200773184555434No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT716730.15796445042804988No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG635190.13999335770428614No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG591090.13027389254463467No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG568540.12530396194712579No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG475150.10472117620427203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC463600.030.8217724
CAGACCG465600.030.7328663
GACCGCG482750.029.5800825
TCAGACC500700.029.0861952
ACCGCGT495750.028.7768946
CTCAGAC567900.026.7576581
CCGCGTT694200.020.5537227
TTCGACG20750.018.4323046
CGCGTTC775050.018.4096438
GCGTTCT833500.017.1537389
CGTTCTC910500.015.87356410
GTTCTCT1018800.014.32309411
CGGTATA31900.014.31855620
CGCGTAA17900.014.0774471
TCGACGA27800.014.0007127
CTCTCCC1081200.013.38299714
CCTCTCA1104100.012.77422419
TCTCCCT1146800.012.72932915
GTTCGAC28350.012.6181965
CTCCCTC1164450.012.4281816
Produced by FastQC (version 0.11.5)