FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45352212
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG2440540.538130312144422No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA993630.21909184936778828No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA814390.17957007256889698No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAA794960.17528582729327513No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG766870.16909208309398446No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC749540.16527088028253176No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG666570.14697629301962162No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG474020.10451970898354417No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT473510.10440725581367455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT276950.025.814721
AGACCGC102250.024.9102544
CAGACCG115300.023.7693253
GACCGCG110600.022.8675755
ACCGCGT111050.022.8150316
CGCGTTC355950.019.9435752
TCAGACC156850.018.7348962
GCGTTCT408350.017.6876983
CGTACGC71900.015.6789785
CCGTACG72350.015.6120614
CGCGTAA16550.015.3649791
CGTTCTC472250.015.3275994
CTCAGAC221650.015.1885191
TCGGCGC76250.014.7245145
TTCGGCG76700.014.52006244
AACGCTA320950.013.99963123
ACGCTAC322400.013.95065624
CGCCGCG80550.013.77092228
TACGCCA83800.013.7207597
TCCGTAC84650.013.6626523
Produced by FastQC (version 0.11.5)