FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47177635
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2224880.47159634008784035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC366450.031.9479124
CAGACCG370000.031.8238243
GACCGCG376400.031.0606445
ACCGCGT384200.030.5165426
TCAGACC398650.030.2404652
CTCAGAC441050.028.7509731
CCGCGTT439700.026.6697867
CGCGTTC466500.025.1085858
GCGTTCT510500.022.979649
CGTTCTC574850.020.759510
CCTCTCA635500.018.18355219
GTTCTCT684350.017.68443511
AATACGG649600.017.5882832
ATACGGA654000.017.4837133
TACGGAG653950.017.37496634
CAATACG665550.017.18029631
ACGGAGA679400.016.78042435
GAACGAT670400.016.76458745
ACTCCCC695550.016.75604825
CCCCAAT690500.016.7386628
Produced by FastQC (version 0.11.5)