FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47177635
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA793420.16817714580224297No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC690060.14626845962075039No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG568570.12051685083408696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29550.016.82492826
AGGGATC135850.013.77078152
ATATAGC50050.013.1300786
TGTCGTT39000.012.92125228
CGTGGCG87750.012.8018021
GTGGCGA90450.012.3200892
TTTCTAT387950.012.1860011
GTCGTTC41850.011.82604829
GGGATCA164000.011.696353
TATAGCT58250.011.5524597
ACGGATC37300.011.4673212
TTGTCGT45200.011.44743827
GGCGACC100350.011.3299534
TTCTATA417150.011.2736212
TGGCGAC102950.011.1750413
CGACCCG103750.011.1714016
GCTAAGT47550.011.16761914
CAGGGAT188750.011.1398061
AAGCCGG270100.011.07650145
TAGGCTA71600.011.03478310
Produced by FastQC (version 0.11.5)