FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43323474
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC656610.15155986798288612No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA572130.13206004670816565No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG494070.11404210105588485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31650.018.76671626
ATATAGC45450.015.79447656
TGTCGTT41050.014.46952828
AGGGATC125100.013.6593422
GTCGTTC43700.013.591928529
CGTGGCG91800.012.6784751
GCTAAGT53000.012.09869714
AATATAG67700.011.9714735
GCGTTCG27550.011.92350315
TATAGCT61100.011.8964057
TTGTCGT52900.011.82387327
TGGCGAC99350.011.8059953
TCGTTCC51600.011.77195430
CGCGTTC28300.011.52792614
CGACCCG101500.011.4845026
GGGATCA150150.011.4551493
GTGGCGA98700.011.4506952
TACAATA64400.011.3267272
CAATATA65400.011.2219954
CAGGGAT170100.011.1965741
Produced by FastQC (version 0.11.5)