FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41682176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG420710.10093283037814532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT67650.017.909051
AGACCGC32900.015.6625714
CCCGTAT24350.014.824741
GACCGCG35450.014.5359265
GTCGGAT48050.014.5087871
CGCGTTC82400.014.1457512
ACCGCGT37550.013.7829256
AATGTCG58600.013.51424943
TAATTAC89400.013.51399125
AACGCTA157900.013.46469623
ACGCTAC160050.013.4243924
TCGTTAC22800.013.41995144
GTAATTA89200.013.26684824
CGGCCTA25000.013.1389228
CGCTACC163250.013.03721525
GCCTACT40200.012.7398971
CTCGGTT63850.012.6852671
CCGGTAT25400.012.5242421
ATGTCGG65950.012.31513844
AAAACGC175350.012.18890621
Produced by FastQC (version 0.11.5)