FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41682176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG133200.012.92771938
GGGAGGT196650.012.3705053
GTTGTCG22250.012.21310526
GGAGGTC198300.012.1448844
GGCATAG143650.011.70098535
AGGGATC111550.011.1417852
TGGCATA156250.010.83760734
GAGCTTA160450.010.63631844
AGCTTAG161600.010.49208945
CATAGGG162000.010.48975437
ACTCTCC225600.010.18890811
GGTCACT229450.010.0120917
GTCACTC229750.09.9510198
GACGCAA41050.09.92721144
AAGCCGG164850.09.77985545
ATATAGC37150.09.688736
GCATAGG177050.09.596971536
TTCTATA260500.09.50764752
CACTCTC237550.09.49423810
TAGGGGA240100.09.468839
Produced by FastQC (version 0.11.5)