FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25077039
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1126050.4490362677986025No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA328580.131028228651716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG200600.031.182943
AGACCGC200800.031.073444
GACCGCG205600.030.303375
ACCGCGT208050.030.0530476
TCAGACC215650.029.584482
CTCAGAC246850.027.161031
CCGCGTT255050.024.4973017
CGCGTTC276200.022.6214228
GCGTTCT302950.020.6759329
CGTTCTC343950.018.47294810
ATACGGA357350.016.99377333
TACGGAG354900.016.9588334
AATACGG358250.016.93851932
ACTCACG45950.016.6969286
CAATACG366400.016.64771831
GAACGAT369300.016.40781445
GTTCTCT391950.016.35991311
ACGGAGA368900.016.33352935
CCCAATA380300.016.16335729
CGGAGAG371850.016.1373936
Produced by FastQC (version 0.11.5)