FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34065795
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2619870.7690617524117667No Hit
CTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA442450.12988101407878488No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC400520.1175724799612045No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG357940.10507313861308683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC430450.033.2269252
CAGACCG429200.032.84133
AGACCGC432800.032.5368844
CTCAGAC455700.032.187261
ACCGCGT447050.031.395676
GACCGCG453700.030.9577855
CCGCGTT494400.028.3975097
CGCGTTC511400.027.4444358
GCGTTCT538300.026.064629
CGTTCTC575400.024.7007810
CCTCTCA580700.023.076419
ATACGGA579400.022.6012633
CCCAATA587900.022.5914829
AATACGG579800.022.58563232
TACGGAG580050.022.40134234
ACTCCCC596450.022.27098525
CAATACG590700.022.25621631
GTTCTCT643500.022.19161811
CCCCAAT599950.022.13375528
GAACGAT585550.022.05317945
Produced by FastQC (version 0.11.5)