FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44962096
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4176280.9288445983479061No Hit
CTCGTCCAACCAGCTGCCACGTCCTGTAACCAAAAGTGACCGGGATGAATG694590.15448345646519682No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA586460.13043431071362865No Hit
CTCACACTCACGCACACTCGCACCGAGACTCAAGGCCGTCTCCACAACTCC471480.10486165947423803No Hit
CTCAGACCGCGTTCTCTCTCTCTCACTCCCCAATACGGAGAGAAGAACGAT468470.10419220669783721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG640150.035.0173763
TCAGACC656750.034.70452
AGACCGC646900.034.613654
GACCGCG657450.034.0368045
ACCGCGT668200.033.4992646
CTCAGAC709050.033.3545841
CCGCGTT752300.029.7572867
CGCGTTC792900.028.2420648
GCGTTCT833700.026.8869299
CGTTCTC900700.025.02195510
GAACGAT918250.024.01592345
AATACGG928600.023.91775132
ATACGGA929550.023.8497933
TACGGAG933050.023.75550534
AGAACGA940500.023.47168544
CAATACG950500.023.37827731
CCCAATA960900.023.18834129
ACGGAGA957650.023.15237435
AAGAACG958650.023.01331943
CCTCTCA875400.022.9771519
Produced by FastQC (version 0.11.5)