FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44962096
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1345150.2991742199918794No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1315600.29260201748601755No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1032100.22954890715059192No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAGG826810.18389044852357417No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG809520.18004498722657414No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG740280.1646453492737527No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG658950.14655677973731474No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG600760.13361476742543318No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA578040.1285616222161885No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT451710.1004646224677782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG41050.023.62119926
ATATAGC50950.021.1590356
AGGGATC161250.019.517412
TATAGCT61500.017.7121947
TGTCGTT55300.017.41225428
CTGGGAG420100.017.0075381
GGGAGGT606950.016.765473
GTCGTTC57150.016.65149529
GGAGGTC608050.016.4392744
GAGGTCA615750.016.3321085
TCGTTCC59600.016.07982630
GTCACTC616500.016.0439558
AATATAG75250.015.97590355
AGGTCAC629000.015.8760816
TGGGAGG419900.015.8640412
CAGGGAT215100.015.6873081
GCTAAGT63300.015.64016514
CAATATA72050.015.5296374
CGTTCGC81000.015.38863916
ACTCTCC648300.015.24786411
Produced by FastQC (version 0.11.5)