FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44809739
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3064980.6839986280660997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC482800.032.503124
GACCGCG480500.032.428155
TCAGACC500500.032.3654442
CAGACCG487400.032.3256233
ACCGCGT489000.032.0186656
CTCAGAC542900.030.9854981
CCGCGTT531350.029.4666947
CGCGTTC544200.028.7667438
GCGTTCT589250.026.624629
GAACGAT603600.025.73666245
AATACGG614150.025.26377532
ATACGGA617350.025.11095233
TACGGAG614950.024.99666434
CGTTCTC648900.024.69377710
AGAACGA635750.024.43833544
CAATACG646400.024.25393531
ACGGAGA639250.024.06061635
AAGAACG651800.023.85401243
CGGAGAG640050.023.70724336
CCCAATA669000.023.68328729
Produced by FastQC (version 0.11.5)