FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42652972
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG35250.020.4250855
AGACCGC38100.019.7244874
ACCGCGT39300.018.6062036
CCGCGTT69250.017.1305221
TCGCGTG5950.015.1270322
CAGACCG51850.014.7980893
CCGGTAT37100.014.3798451
CGCGTTC81700.014.3216152
AACGCTA133050.014.12057123
ACGCTAC134050.013.99846524
CGCTACC136250.013.68986625
CGCGTAA13650.013.5226241
GCGTACG8550.013.42099723
CCGTACG65750.013.346064
CGTACGC66950.013.1737235
GCCTACG22050.013.16293210
CGTGGTT140150.012.87578241
ACTCACG74850.012.7149736
CCCGTAT29550.012.4929821
AAAACGC153150.012.35517721
Produced by FastQC (version 0.11.5)