FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45907972
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1668510.36344667980541595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG264800.032.6976055
AGACCGC265750.032.682894
CAGACCG268550.032.375643
ACCGCGT267800.032.295526
CCGCGTT294150.029.4485727
TCAGACC307100.028.9449582
CGCGTTC306300.028.236368
CTCAGAC366350.026.6277481
GCGTTCT343450.025.3131379
AATACGG344550.023.86784432
ATACGGA351400.023.41543433
TACGGAG352300.023.25996834
GAACGAT354450.023.04247945
CAATACG362200.022.86627631
CCCAATA373000.022.28258929
ACGGAGA375100.021.86418235
AAGAACG375000.021.78575143
AGAACGA377650.021.63285644
CGGAGAG381950.021.39548536
CCAATAC400350.020.96266730
Produced by FastQC (version 0.11.5)