FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45907972
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAG45150.011.01152917
TCGATAA45950.010.96739716
AGGGATC120650.010.540272
TAAGTCG43150.010.47906920
GTTGTCG22950.09.9024126
TTCTATA246100.09.8774022
TTTCTAT250100.09.8184631
TCTATAG238650.09.5068323
TTCGATA53550.09.45329215
CAGGGAT175050.08.7562361
GGGATCA146450.08.683523
GACGCAA55100.08.6145644
CTATAGT280300.08.3832021
TGTCGTT28600.08.33904728
CTTGAAC236250.08.2885751
ACGCAAG56850.08.27025745
ATAAGTC60050.08.20405619
TACGTAT27300.07.99249643
GCGTAGG25400.07.97302836
CGTAGGG25900.07.90592737
Produced by FastQC (version 0.11.5)