FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43844216
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG692890.15803452843129867No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC484340.1104683910872075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC116300.017.619395
TCACCCG127950.016.7984943
ATAGCTC127000.015.38420339
GGAGGTC372650.014.9849694
AGGGATC141400.014.9549742
GGGAGGT392600.014.74045853
GTTGTCG22450.014.70367226
ACACGGA136450.014.60911216
GGCATAG240350.014.57897735
ACTCTCC377450.014.4587611
GTCACTC381400.014.2254448
ATAGGGG248100.014.1804938
CACCCGG150300.014.1101944
AGCTTAG246900.014.04449745
CGGACAC144350.014.04041513
GGTCACT390800.013.927867
TAGCTCT140500.013.92204340
TGGCATA253550.013.86257534
GACACGG147500.013.743587515
AGGTCAC404100.013.7405566
Produced by FastQC (version 0.11.5)