FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43844216
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT578650.13197864001034937No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG455440.10387687169500306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC102950.029.3990254
ACCGCGT104950.028.5600346
CAGACCG115200.026.5267643
GACCGCG113100.026.48215
TCAGACC163500.020.2869472
TTCGGCG90950.020.23461544
CCGCGTT147350.020.2306617
CCGTACG99350.018.8901394
ATGCGCG39100.018.87302244
CGTACGC100350.018.6121985
TCGGCGC101150.018.26093945
TGCGCGT41500.017.94426745
CGCGTTC166300.017.8983068
CGCGTAA45800.017.8337141
CTCAGAC221250.017.7955271
TCCGTAC106850.017.6695063
TACGCCA113850.016.579577
TTCGCGC26850.016.506937
GTACGCC115050.016.3905816
CGCCCCG121200.015.36993723
Produced by FastQC (version 0.11.5)