FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38326857
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2692190.702429108653496No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT1232280.32151866770604226No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA633270.1652287846091841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG553450.035.101843
TCAGACC561100.035.0002562
AGACCGC559000.034.692364
CTCAGAC593650.034.454171
GACCGCG572500.033.786235
ACCGCGT583850.033.1489146
CCGCGTT672000.028.7935527
ATACGGG213950.027.93157633
TACGGGG220200.027.06715434
CGCGTTC723450.026.752018
GCGTTCT765000.025.3372429
CGTTCTC818550.023.80060810
ACGGGGA253650.023.70182635
GTTCTCT890550.021.97235711
CGGGGAG278800.021.5559736
AATACGG889650.021.31751332
CCTCTCA900750.021.21157519
CCCAATA914650.020.87238529
CAATACG911850.020.83571831
CCCCAAT925200.020.6709128
Produced by FastQC (version 0.11.5)