FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41239194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA437590.10611022126184134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG103050.014.8538981
GGGAGGT250400.013.2598093
GGAGGTC246800.013.1521894
GTGGCGA116900.012.708932
GTCACTC243550.012.6422188
GGTCACT244500.012.5668977
CGACCCG122950.012.3031136
TGGCGAC124500.012.1139283
GGCGACC129500.011.6287844
AGGTCAC277000.011.5312636
ATAGGGG175400.011.51710838
ACTCTCC268600.011.3450611
GCGACCC133600.011.3224595
GGCATAG180750.011.15132235
GCGTAGG96650.011.10230636
GACGCAA41200.011.08397444
GGCGTAG96850.011.0793835
GAGGTCA296900.011.016245
CACTCTC278150.011.01240510
CGTAGGG97600.010.97119237
Produced by FastQC (version 0.11.5)