FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26192705
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1371450.5235999870956436No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA275450.10516286882168145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC219500.032.7509354
CAGACCG221450.032.656093
GACCGCG221900.032.3358735
TCAGACC236250.031.1074852
ACCGCGT230800.031.0781966
CTCAGAC267750.029.3629681
CCGCGTT265850.026.980867
CGCGTTC279350.025.5239378
GCGTTCT308250.023.1674359
CGTTCTC342100.021.17760710
AATACGG360250.019.49899332
ATACGGA363150.019.35574333
TACGGAG364050.019.2769934
CAATACG373250.018.88613131
CCCAATA375500.018.8804629
GTTCTCT390500.018.78324711
GAACGAT372250.018.71326345
CCTCTCA382450.018.54905319
ACGGAGA378350.018.51877435
CCCCAAT389900.018.26971428
Produced by FastQC (version 0.11.5)