FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29949619
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT5461021.8234021608087903No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA694990.23205303546599376No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA472180.15765809908967457No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT337580.11271595808948355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC707100.037.5402372
CTCAGAC743400.036.6174551
CAGACCG722350.036.5403983
AGACCGC732200.035.9717754
GACCGCG746450.035.2579355
ACCGCGT761350.034.558536
CCGCGTT845400.031.125637
CGGTATA55150.030.63853820
TTCGACG42950.030.3837476
CGCGTTC889900.029.5843438
GCGTTCT919150.028.7040799
TCGACGA46350.028.203737
CGTTCTC958850.027.6399910
GTTCGAC47800.026.877655
GTTCTCT1020450.026.0486611
AATACGG995150.026.03722632
ATACGGA995000.026.01866733
TACGGAG995750.025.9448434
CCTCTCA1014000.025.77905719
GAACGAT998950.025.77375845
Produced by FastQC (version 0.11.5)