FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29949619
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1689940.5642609343377624No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1126900.3762652206026394No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG685170.22877419575855037No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA608730.20325133351445976No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT605960.20232644695747215No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG586100.19569531084852868No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC566100.18901742957063997No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG481770.1608601431624222No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA335700.11208823724936201No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA306330.10228176859278244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG25550.022.80803326
AGGGATC136550.021.4585652
ATATAGC34750.019.361956
CAGGGAT164700.018.8974291
TGTCGTT32100.018.50402628
TTGTCGT34650.017.46724327
GGGATCA171900.017.3862723
TATAGCT41850.016.1310847
GTCGTTC36350.016.09187129
TCGTTCC38500.015.42682230
GGATCAT194250.015.2816234
GCTAAGT39850.015.1882114
AATATAG50100.014.7794325
AACTCTA96100.014.25506145
TATATAC41350.014.2037447
CAATATA50400.014.0211894
TACAATA51750.013.7857472
CGTTCCC44350.013.44516531
CGTGGCG66450.013.3436721
TAGGCTA61000.013.3156210
Produced by FastQC (version 0.11.5)