FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53899036
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG933260.17314966449492714No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC851000.15788779598952382No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA771360.143112021521127No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT700840.1300282995784934No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA550670.10216694784671103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG37650.020.01707626
AGGGATC184950.015.6046932
ATATAGC57650.015.0683976
TGTCGTT51350.014.28230928
GGGATCA227550.013.1295173
CAGGGAT239700.013.1204241
GTCGTTC57400.013.09020729
TTGTCGT60600.013.06734227
AAGCCGG375000.012.61512945
GCTAAGT62200.012.37206314
AATATAG86500.012.2078525
GCGTGAA398550.012.1056240
TCGTTCC62200.012.07995230
TATAGCT73850.012.068077
CAATATA76400.011.8762474
GCGTTCG33650.011.8351915
ATAGGGG145200.011.773972538
AGCGTGA408900.011.7444839
TTTCTAT364000.011.6086681
TTCTATA367900.011.2286582
Produced by FastQC (version 0.11.5)