FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50796902
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT7642871.5045937250267742No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA944180.18587354008321216No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA793330.1561768471628447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC1058100.036.83842
CTCAGAC1104400.036.0363921
CAGACCG1077100.035.815083
AGACCGC1096550.035.154534
GACCGCG1106850.034.737575
ACCGCGT1129150.034.0958486
CCGCGTT1243950.030.9655347
CGCGTTC1292950.029.804198
GCGTTCT1349350.028.5934529
CGTTCTC1428850.027.21495810
AATACGG1504950.025.16024432
CCTCTCA1528400.025.13615419
GTTCTCT1553850.025.13568911
ATACGGA1506400.025.11364633
TACGGAG1503000.025.03572734
GAACGAT1500150.025.03536845
CAATACG1527800.024.85168631
CCCAATA1540550.024.79624429
ACTCCCC1546550.024.77135525
ACGGAGA1533750.024.5470435
Produced by FastQC (version 0.11.5)