FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35759929
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN725800.2029646087943855No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG359250.10046160885833974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC103800.014.5294222
GGGAGGT245650.013.2491963
GTCACTC238700.013.1596868
TTCTATA266900.012.8109142
GTTCTAT162600.012.6688521
ACTCTCC245650.012.61258511
GGAGGTC256050.012.5788424
CAGGGAT144450.011.8605231
CACTCTC270800.011.59911710
GGGATCA133850.011.4516153
GGCATAG155900.011.213057535
TCTATAG299950.011.1958723
TCACTCT291900.011.1077319
GGTCACT279700.011.0698447
AGGTCAC290500.011.030136
AGCATAG125100.010.95516145
ATAGGGG165300.010.7123638
AGCTTAG161100.010.65829745
GACGCAA36550.010.65108344
CGTCGAT16150.010.58832812
Produced by FastQC (version 0.11.5)