FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40037876
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1228520.30683945372127136No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCA404100.1009294299228061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT91300.023.2571321
AGACCGC38250.019.295594
GACCGCG40600.018.1233045
CGCGTTC121500.017.1864952
ACCGCGT43000.017.111776
CAGACCG44800.017.0771963
CGCGTAA16100.015.9777751
CCGTACG55350.015.0824534
GCCTACG19850.014.62092610
CTCGGTT64150.014.5978441
CGTACGC57400.014.4653895
CCGGTAT29550.013.7452391
GCGTTCT165950.013.4915383
CGGCAGT477550.013.17708440
ATGCGCG13950.012.90213144
CCCGTAT20950.012.8174091
TACGCCA67150.012.7651927
GCGTACG6350.012.75482423
AACGCTA131450.012.69956923
ACGCTAC130650.012.67401124
Produced by FastQC (version 0.11.5)