FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40037876
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA630220.15740595230376356No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC541260.13518699143780755No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG412210.10295501189923262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC128300.015.852462
ATAGGGG139950.015.71643438
GGCATAG140900.015.68106735
GGGAGGT226000.015.6390773
GGTCACT238700.014.3756097
GTTGTCG19450.014.20133526
AGCTTAG155300.014.17791145
GTCACTC247650.014.07468
GGAGGTC253900.014.0127824
TGGCATA161350.013.85221434
GAGCTTA165450.013.4708144
TATCCTA106200.013.1523265
AGGTCAC269750.013.0343216
CATAGGG172300.012.88241637
ACTCTCC276450.012.8418911
GAGGTCA281000.012.807055
CAGGGAT177450.012.8057421
GGGATCA162100.012.7744913
ATCCTAG116500.012.7054726
TCACTCT285500.012.565129
Produced by FastQC (version 0.11.5)