FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33599376
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG905850.2696032212026795No Hit
TTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGC818650.24365035826855835No Hit
CTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA799400.2379210852011061No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCC619030.18423854062051628No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGG617840.1838843673763465No Hit
CCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG565010.1681608610826582No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC472390.14059487295240244No Hit
CTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGGA460070.13692813818923305No Hit
GTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGT437830.13030896764273242No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG413540.12307966671761998No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC401570.11951710055567699No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG395430.11768968566559093No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC389640.11596643937673128No Hit
CCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGC389470.11591584319899276No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC385120.11462117629803602No Hit
CGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGGAG375090.1116360018114622No Hit
CTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCC361950.1077252148968481No Hit
CCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGC354550.10552279304234698No Hit
CTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGC352260.10484123276575136No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA344700.10259119097926105No Hit
TGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGC340010.10119533172282724No Hit
CGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGC339730.1011119968418461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT11500.026.0948431
AGACCGC50700.021.525384
CAGACCG56250.019.761573
CCGCGTT107100.019.1292211
ACCGCGT61600.017.6069186
CGCGTAA11500.016.8733561
ATTGAGT119250.016.057885
CGCGTTC128100.015.8794812
GGTTTAT86650.015.6236881
TATTGAG127400.015.2602394
GAGTGCA138700.015.0690488
TGCATCG58050.015.03751518
CGTACGC39500.014.9822035
GCGTTCT139900.014.8778793
GCCTACG10000.014.84877110
CCGTACG39650.014.8120214
ATCGTAG61850.014.76838221
TCCGTAC40400.014.7041383
CCGGTAT21350.014.6898651
GTTTATT151100.014.6339811
Produced by FastQC (version 0.11.5)