FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33599376
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA680100.20241447341164906No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTG646960.1925511949983833No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG531740.1582588914746512No Hit
CTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGG496460.1477586964710297No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA419370.12481481798947695No Hit
GTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCT415040.12352610358001886No Hit
CTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAA402600.11982365386785754No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC385180.11463903377253197No Hit
CCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGT383820.11423426435062366No Hit
CTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTC382180.1137461600477342No Hit
CCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCG367040.10924012398325492No Hit
CAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTA344310.10247511739503734No Hit
CAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGA341790.10172510346620724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGG29950.014.1173234
GCGTGAA118150.013.24200540
CGTGGCG60100.012.9647761
GGGAGGT125750.012.9276233
TTAGCCC43200.012.9016522
AAGCCGG118550.012.87468645
GTGGCGA60050.012.6497252
GTCAGGA115150.012.4998311
GGAGGTC124950.012.4555014
ATAGGGG89600.012.41112838
ACGCGCG16650.012.31088220
CGACCCG63250.012.2702716
GGCGACC63950.011.9220374
GGACACA350100.011.8521074
CGTAACG5900.011.82802942
TGGCGAC65250.011.8195783
GGGATCA103550.011.790663
CCAGGAC383400.011.7486731
TTTCTAT259750.011.7309811
GGCATAG91300.011.7303835
Produced by FastQC (version 0.11.5)