FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43542014
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC959080.22026541996886043No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA683300.15692889171364466No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG567020.13022365019679613No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG508880.11687102943837187No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT479410.11010285376326415No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC446890.10263420520695253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29550.019.11094526
AGGGATC133200.016.3088112
TGTCGTT35900.015.91886728
ATATAGC41550.015.6513086
GGGAGGT315100.014.1092653
TATAGCT45300.014.0082787
GCGTTCG42550.013.80121515
GTCACTC320100.013.3359968
CAGGGAT181900.013.3280531
ATAGGGG217200.013.19564238
AGCTTAG222650.012.86119345
GGGATCA174950.012.68673
GGAGGTC345150.012.6785644
CGTTCGC47050.012.671926516
GGCATAG227600.012.6245135
GCTAAGT45800.012.47793214
CGCGTTC47450.012.4233714
CGTCGAT22750.012.36233412
TTGTCGT46150.012.18845527
TGGCATA241300.012.0660734
Produced by FastQC (version 0.11.5)