FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43542014
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4237760.9732576908362576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC674550.035.3641742
CAGACCG666600.035.2268753
AGACCGC675700.034.711984
GACCGCG684600.034.1485755
ACCGCGT694550.033.7034076
CTCAGAC738450.033.3877331
CCGCGTT747300.031.327387
CGCGTTC765050.030.5946678
GCGTTCT829050.028.3549829
CGTTCTC876000.026.9713410
GAACGAT824900.025.97214745
AATACGG831550.025.91040632
ATACGGA831650.025.9072933
ACTCCCC841700.025.85448625
TACGGAG834800.025.71250534
CAATACG841850.025.62546531
AGAACGA842850.025.44023144
AAGAACG852650.025.1293643
CCCAATA860700.025.11900129
GTTCTCT954200.024.89299211
Produced by FastQC (version 0.11.5)