FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46814985
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA18850.016.8320061
CCGTACG41750.016.1134684
AGACCGC36750.016.0405144
CGTACGC45000.015.04975
GACCGCG39100.014.7311645
CGCGTTT11950.014.4994321
CCCGTAT31950.014.2268471
AACGCTA132900.014.103497523
ACGCTAC134500.013.952661524
CGCTACC138950.013.44083825
CCGCGTT68500.013.2714681
ACCGCGT44450.013.1099476
CAGACCG47150.012.7886353
CTCGTAT65000.012.6359421
TACGCCA56100.012.4729877
CCGCAAT35350.012.4129031
CTCCGAT43750.012.3955881
CCCGGAT112350.012.3177161
AAAACGC156250.012.12453321
GAAAACG156400.011.94027420
Produced by FastQC (version 0.11.5)