FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38116688
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA33750.018.6621138
GCCTACG33650.018.51702710
TCGTTAC29800.016.75846944
CCTACGT39050.016.6484311
CCGCGTT32950.015.7639221
CGGAAAT51600.015.668421
CTACGTC42800.015.34748712
AACGCTA116700.014.61082923
ACGCTAC117950.014.60855924
CGCTACC118450.014.375977525
TACGTCC45250.014.37082613
AATCGGC48650.014.3425035
GCCGGCT126900.013.9701121
GAAATCG51950.013.908033
TTCGACG12650.013.8787456
ATCGGCC50450.013.8307786
GTGTAAT113050.013.37135122
GTAATTA114450.013.22744124
AAATCGG54400.013.1575284
TAATTAC118250.013.14478425
Produced by FastQC (version 0.11.5)