FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38116688
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500590.13133092780778854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG68350.016.9960271
CGACCCG84500.013.5337326
TGGCGAC89200.013.1237413
GTGGCGA87700.013.091512
TACGGTT46750.012.94374235
GGCGACC89350.012.8748634
CGTAACG12400.012.69888342
GCGACCC93350.012.4437625
TTACGGT50500.011.71530334
GGGAGGT161000.011.2840973
GGAGGTC155050.011.2379094
TGTTACG53650.011.237132
ACCCGCT111600.010.8886148
GACCCGC112100.010.761267
ACGGTTG55250.010.70810536
CGGTTGG56750.010.54398937
ATAGGGG109400.010.507339538
GTTACGG59850.010.26095233
AACTAAC160700.010.05073337
GCGTAGG54650.09.92009936
Produced by FastQC (version 0.11.5)