FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46425978
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1329340.28633537886913224No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG757280.163115572923418No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT750640.161685339186608No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT672400.144832705516726No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG633600.1364753156088602No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG577600.12441310337070335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC142950.026.392444
TTCGACG27900.025.319126
CAGACCG151250.025.0638523
GACCGCG160950.023.5941935
CGGTATA41400.022.9335520
ACCGCGT168900.022.4036876
CCGCGTT336400.021.392461
TCGACGA33650.020.9926747
TCAGACC204850.019.8786032
GTTCGAC37850.019.138755
CGACGAG38600.018.067488
CTCAGAC255100.017.8805771
CGCGTTC437400.016.3256862
GCCTACG22050.015.20200410
GCGTTCT487450.015.0047993
GTATAGA67050.014.86537422
AACGACG26700.014.40814316
AGTTCGA52350.014.3965624
CGGTATG36500.014.36238121
CCGGTAT35550.013.9891361
Produced by FastQC (version 0.11.5)