FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29505156
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1096430.3716062372285034No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG409800.13889097891907434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG190450.031.2368585
AGACCGC194350.030.911254
CAGACCG195350.030.891653
ACCGCGT194700.030.635236
TCAGACC219200.028.2403952
CTCAGAC239300.027.5474451
CCGCGTT225450.026.4767747
CGCGTTC239300.024.9537758
TTCGACG12800.022.675516
GCGTTCT266450.022.48719
CGTTCTC303700.020.09939810
AATACGG313450.018.24686632
ATACGGA313850.018.1591233
TACGGAG312800.017.97544934
GAACGAT320150.017.86500245
CAATACG329850.017.66702831
CCTCTCA342650.017.45987919
CCCAATA345400.017.22324429
CGGAGAG325900.016.9423136
ACGGAGA332350.016.91815835
Produced by FastQC (version 0.11.5)