FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48476082
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3839590.7920586486341863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG593200.033.530783
AGACCGC594850.033.4146964
TCAGACC610850.032.958282
GACCGCG614200.032.281335
ACCGCGT624450.031.808516
CTCAGAC671450.031.1408751
CCGCGTT679450.029.2304277
CGCGTTC695450.028.5579328
GCGTTCT725500.027.3998819
CGTTCTC794100.025.3133910
GAACGAT751300.025.27624945
AATACGG764450.025.16495332
ATACGGA766450.025.05239533
TACGGAG766300.024.91641434
CAATACG782950.024.64785631
CCCAATA805500.024.17830729
AGAACGA787650.024.16082244
ACGGAGA790950.024.15990435
AAGAACG799400.023.79436143
CCTCTCA827700.023.73907319
Produced by FastQC (version 0.11.5)