FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40465475
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2293620.5668091132008212No Hit
CTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA654550.16175517524507002No Hit
GTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGA478840.11833297397349221No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC468540.1157875942392867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC355650.032.8483284
CAGACCG359150.032.6853643
ACCGCGT366150.031.8801846
TCAGACC376600.031.8169772
CTCAGAC425000.029.8654351
CCGCGTT394650.029.560797
GACCGCG408250.028.57725
CGCGTTC408000.028.5273698
GAACGAT415100.027.63842245
AATACGG429150.026.7073532
ATACGGA430200.026.67884333
GCGTTCT439350.026.563489
TACGGAG430050.026.5311934
AGAACGA434300.026.44734444
CAATACG440650.026.19416631
AAGAACG448400.025.6107243
ACGGAGA452100.025.27206835
CCCAATA464400.025.09159329
CGGAGAG454200.024.99190136
CGTTCTC487550.024.19092210
Produced by FastQC (version 0.11.5)