FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51428933
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1079370.2098760244549503No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA965510.18773673566200566No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG603830.11741056342739989No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG597620.11620307191673605No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT589640.11465141615907139No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA523590.1018084508966966No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG522390.10157511920381472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG37300.019.48097426
ACCGGTA133100.017.15380945
ATATAGC48050.016.908436
TACCGGT138750.016.4540644
GTGTCGC144100.016.33565312
TGTCGTT46200.015.72825228
AGGGATC160600.015.5882782
GTTACCG157500.014.80929742
TTACCGG161000.014.5433243
GTCGCCA165200.014.24736714
GCGTTCG43650.014.07256115
CCGTTGT164450.014.00771837
TGTCGCC169500.013.99315413
GGGAGGT363150.013.7576953
GCCCGTT172700.013.28657235
GCTAAGT57650.013.19259814
CCGCGTT47500.013.17030613
CGCGTTC47850.013.12004214
GGAGGTC377750.013.1009224
ATAGGGG219650.012.99567338
Produced by FastQC (version 0.11.5)