FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42166040
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1018490.24154272016058423No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA819720.19440288914965692No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG599030.14206456190811373No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT513070.12167848818622759No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG471170.11174158161401924No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA446290.10584109866613037No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC445610.1056798314472974No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG426300.10110031674779041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG37000.022.49476626
TGTCGTT42150.019.90635128
ATATAGC49100.018.9797486
TATAGCT54100.017.4749897
AGGGATC141050.017.384472
TTGTCGT50200.016.75900827
GCTAAGT55450.015.78531114
TCGTTCC52900.015.69010630
GTCGTTC52600.015.65158329
TACAATA62050.015.5676192
AATATAG72300.015.135815
GGGATCA179400.014.3343223
CGTTCCC58400.014.32890731
CAGGGAT189350.014.30522251
GCGTTCG31450.014.09366215
CAATATA71000.014.081434
TAAGTGC62700.013.70741616
CTAAGTG65550.013.28362515
GGATCAT197150.012.83783154
CGTCGAT23350.012.7211612
Produced by FastQC (version 0.11.5)