FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49322154
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT6462591.3102813798440351No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA663900.13460482686948344No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA608090.12328942486980597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC943200.035.284862
CTCAGAC989850.034.4415171
CAGACCG961200.034.2915653
AGACCGC965400.034.102444
GACCGCG997850.032.9294555
ACCGCGT1004850.032.7225726
CCGCGTT1084450.030.299617
CGCGTTC1112600.029.528568
GCGTTCT1152450.028.5328889
GAACGAT1170300.027.25371445
CGTTCTC1235550.026.8578510
AATACGG1217400.026.49807232
ATACGGA1220600.026.40650733
TACGGAG1217250.026.36645334
AGAACGA1215300.026.35208544
CAATACG1233400.026.14663531
ACGGAGA1238800.025.91857535
AAGAACG1241050.025.786443
CCCAATA1260350.025.70151129
CCTCTCA1274800.025.6248319
Produced by FastQC (version 0.11.5)