FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49322154
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1355950.2749170281573672No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1191480.24157095815401736No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA719530.1458837341126667No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG703310.14259515105524387No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT671250.13609502942633042No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG662030.13422568689923803No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG658650.1335403964717356No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC495860.1005349441956651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC227550.019.3246172
CAGGGAT272800.017.0595471
GTTGTCG26650.016.6287926
GGGATCA275750.016.1601313
GCGTTCG63950.015.65647115
CGCGTTC66300.015.40779614
CGTTCGC65900.015.22674716
CCGCGTT68300.015.22060113
GGATCAT268650.013.4102734
TTCGCAC76650.012.9728318
ATATAGC42350.012.8626566
AGTTAGG40550.012.7714864
ATAGGGG175150.012.61251738
GGGAGGT306600.012.5952443
GGAGGTC297450.012.5054854
TGTCGTT36300.012.39409928
GTTCGCA82500.012.35327517
GCCGCGT85300.012.26695912
GGCATAG181100.012.1609235
TCGCACC83350.012.11839619
Produced by FastQC (version 0.11.5)